Subject Area

Biology

Description

Cassandra Phillips ’20
Major: Biology and Psychology

Dak Ojuka '20
Major: Biology Minor: Business and Innovation

Anne-Deyssa Victor '21
Major: Biology

Ramon G. Ortega-Alvarez ’20
Major: Biology

Kathryn Fama ’21
Major: Biology

Faculty Mentor: Dr. Charles Toth, Biology

The purpose of this experiment was to develop an in vitro model of the blood brain barrier that incorporated brain endothelial microvascular cells (BMEC) and co-cultures composed of neurons, astrocytes, and macrophages. All cell types were derived from human induced pluripotent stem cells. A transendothelial electrical resistance (TEER) assay and immunofluorescence were used to evaluate barrier function. In addition, the model was used to investigate acute neuro-inflammatory responses. An in vitro model of the blood brain barrier could be a valuable tool to observe and assess neuro-inflammation because a human stem cell model is more physiologically relevant.

Publisher

Providence College

Date

4-22-2020

Start Date

4-22-2020 12:00 AM

Type

Poster

.pdf

Language

English

Included in

Biology Commons

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