Veronica Bohl ’20
Major: Biology and Psychology, Neuroscience Certificate Program
Nicholas Dash ’20
Faculty Mentor: Dr. Charles Toth, Biology
This experiment provides insight on how using metabolites derived naturally from the microbiome can influence cardiac inflammation.3D cardiomyocytes were derived from human induced pluripotent stem cells through the utilization of small molecules and growth factors. Quantitative PCR was performed to verify that the organoids expressed genes associated with cardiomyocyte cells. Primers for the genes FOXa2, TNNT2, MYH7, AHR, CACNA1, and KCNH2 were used for the qPCR, as these genes are expressed more prominently in cardiomyocyte cells than the undifferentiated iPSCs.Cardiovascular disease was modeled by inducing inflammation in the organoids using lipopolysaccharide (LPS), a gram-negative bacteria membrane product associated with inflammation and septic shock, indoxyl-3-sulfate (I3S), which is representative of uremic toxins build-up, and poly (I:C), which simulates a viral infection. Cell viability was analyzed by a luminescence (Cell Titer Glo) assay.
4-22-2020 12:00 AM