Subject Area
Chemistry
Description
Colby Agostino ’22
Major: Biochemistry and Computer Science
Olivia Schmitt ’22
Major: Biochemistry
Faculty Mentor: Dr. Kathleen A. Cornely, Chemistry and Biochemistry
ZoeJ and Marcoliusprime are mycobacteriophage capable of infecting tuberculosis causing bacteria via the lytic and lysogenic cycles. The lytic cycle is the form of viral replication that results in cell death. Lysogeny involves phage DNA being integrated with the DNA of the host cell and preserved through binary fission. However, the bacteria causing tuberculosis do not die in the lysogenic phase. This research project will involve creating mutants of ZoeJ and Marcoliusprime that will not contain the genes facilitating lysogeny. The mutation of the phage genomes will be accomplished via a BRED, bacteriophage recombineering with electroporated DNA.
Publisher
Providence College
Date
4-22-2020
Start Date
4-22-2020 12:00 AM
Type
Presentation
Format
Video
.mp4
Language
English
