Faculty Mentor: Dr. Kathleen Cornely, Chemistry and Biochemistry
αβ-Carbonic anhydrases are metalloenzymes that are essential to the growth of bacteria, making them a target for antibiotic research in the drug industry. These enzymes contain zinc ions that catalyze the hydration of carbon dioxide to bicarbonate for homeostatic balance. To enhance isolation, surface mutations were completed to introduce histidine residues because they demonstrate affinity for Ni-NTA resin in a column chromatography procedure. After generation and isolation of these mutants, the mutant HICA proteins can be used in a kinetic assay to observe the effect of mutation on the rate of reaction.
4-22-2020 12:00 AM